Genetic engineering service (SIG)


Skills, know-how


Strain collections

  • Keio collection: E. coli BW25113 (wt = IIh9). 3985 single-gene KO mutants. essential genes + undisrupted strains. Reference.
  • SPA (Sequential Peptide Affinity) tag collection: E. coli DY330. C-term affinity-tagged (SPA) alleles of 857 open reading frames for bacterial interactome. Reference.
  • ASKA (-): E. Coli AG1. N term His tagged alleles of 4267 open reading frames on plasmid – high copy number – CmR – IPTG inducible. Reference.


  • 2-Lipoprotein DolP supports proper folding of BamA in the bacterial outer membrane promoting fitness upon envelope stress. Ranava D, Yang Y, Orenday-Tapia L, Rousset F, Turlan C, Morales V, Cui L, Moulin C, Froment C, Munoz G, Rech J, Marcoux J, Caumont-Sarcos A, Albenne C, Bikard D, Ieva R. Elife. 2021 Apr 13;10:e67817. Pubmed 33847565 DOI: 10.7554/eLife.67817
  • 1- Addressing the role of centromere sites in activation of ParB proteins for partition complex assembly. Audibert S, Tanguy-le-Gac N, Rech J, Turlan C, Bouet JY, Bystricky K, Lane D. PLoS One. 2020 May 7;15(5):e0226472. Pubmed 32379828 DOI: 10.1371/journal.pone.0226472


Research projects:

  • Catherine TURLAN Research Engineer CNRS, head of the service. Office 316 – phone 05 61 33 58 80 – email.
  • Anne-Marie CIRINESI Technician CNRS (50%). Office 315 – phone 05 61 33 59 79 – email.


Team – office 317 – Email